As a new tool in CRISPR genome editing,Cpf1 has sparked an explosion of interest for its attributes that differ from Cas9: It requires only a single RNA that CRISPR RNA assembly is simpler; its staggered cleavage patterns may facilitate substituting existing DNA with desired sequences; and it recognizes thymidine-wealthy DNA sequences, which has been less explored than the guanosine-wealthy sequences recognized by Cas9. In sum, or Cpf1 is expected to broaden the scope of CRISPR genome editing target sites with enhanced efficiency. Despite Cpf1's vast potential as a powerful genome editing tool,exiguous has been demonstrated as to how, specifically, and the new tool finds its targets. In a series of two papers published online on June 6 in Nature Biotechnology,researchers at the IBS middle for Genome Editing in South Korea showed Cpf1 as a highly specific programmable tool that is suitable for precision genome editing and reported generation of mutant mice using CRISPR-Cpf1.
Source: phys.org